Acta Limnologica Brasiliensia
https://actalb.org/article/doi/10.1590/S2179-975X2721
Acta Limnologica Brasiliensia
Methods

Simple methodological approach for assessing microbial mineralization rates in an aqueous anaerobic medium

Abordagem metodológica simples para avaliação das taxas de mineralização microbiana em meio aquoso anaeróbio

Marcela Bianchessi da Cunha-Santino; Irineu Bianchini Junior

http://dx.doi.org/10.1590/S2179-975X2721 Acta Limnol. Bras. (Online), vol.33, e202, 2021
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Abstract

Abstract:: The aim of this study was to propose and discuss a simple manometric method to quantify the emission rates of gases resulting from the microbial anaerobic mineralization of organic resources, as leaves, thin branches, and macrophyte detritus. The proposed method can be used under laboratory conditions. The method consists of using a water pressure gauge attached to the reaction flask. The incubations were prepared with samples of water from Paranapanema River and Typha domingensis, the experiment lasted 9.8 months. The procedures for preparing the incubations are presented in detail, as well as the calculations for the conversion of the volumetric measurement into carbon mass (i.e., daily rate of carbon gas emissions). According to the results obtained from T. domingensis mineralization assays it was possible to demonstrate that, numerous events related to mineralization could be adequately addressed (e.g., the heterogeneous detritus composition). The results of this method were quite convergent with those obtained in kinetic experiments (used as a reference) after the 30th mineralization day, suggesting the use of this method mainly for medium- and long-term experiments. As exemplified by T. domingensis incubations, this method is particularly valuable for the systemic comparison of the several organic resources mineralization and for the primary measurement of the main parameters involved (e.g., reaction rates constants). This method combined with other short-term experiments can greatly improve the understanding of the cycling of organic resources in aquatic environments.

Keywords

water pressure gauge, decomposition, carbon cycling, aquatic environment, greenhouse gases

Resumo

Resumo:: O objetivo deste estudo foi propor e discutir um método manométrico simples para quantificar as taxas de emissão de gases resultantes da mineralização anaeróbia microbiana de recursos orgânicos, como folhas, galhos finos e detritos de macrófitas. O método proposto pode ser usado em condições de laboratório. O método consiste na utilização de um manômetro de água acoplado ao frasco de reação. As incubações foram preparadas com amostras de água do rio Paranapanema e Typha domingensis, o experimento durou 9,8 meses. Os procedimentos para a preparação das incubações são apresentados em detalhes, bem como os cálculos para a conversão da medição volumétrica em massa de carbono (ou seja, taxa diária de emissões de gás de carbono). De acordo com os resultados obtidos da mineralização de T. domingensis foi possível demonstrar que, inúmeros eventos relacionados à mineralização podem ser adequadamente tratados (por exemplo, a composição heterogênea dos detritos). Os resultados deste método foram bastante convergentes com os obtidos em experimentos cinéticos (usado como referência) após o 30º dia de mineralização, sugerindo a utilização deste método principalmente para experimentos de médio e longo prazo. Como exemplificado com incubações de T. domingensis, este método é particularmente valioso para a comparação sistêmica da mineralização de vários recursos orgânicos e para a medição primária dos principais parâmetros envolvidos (por exemplo, constantes de reação). Este método combinado com outros experimentos de curto prazo pode melhorar muito a compreensão da ciclagem de recursos orgânicos em ambientes aquáticos.
 

Palavras-chave

método manométrico, decomposição, ciclo do carbono, ambiente aquático, gases de efeito estufa

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Submitted date:
04/06/2021

Accepted date:
08/16/2021

Publication date:
09/13/2021

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