Acta Limnologica Brasiliensia
https://actalb.org/article/doi/10.1590/S2179-975X11323
Acta Limnologica Brasiliensia
Thematic Section: Methods

Comparative analysis of ex situ zooplankton hatching methods

Análise comparativa de métodos de eclosão de zooplâncton ex situ

Daniel Nino Flores-Mendez; María Florencia Gutierrez

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Abstract

Aims: This study aims to analyze the efficiency of two novel methods for ex situ zooplankton hatching experiments, compared with a traditional one. Both proposed methods were specifically designed to minimize sediment resuspension during the sampling of hatched individuals when no previous egg isolation is performed.

Methods: Sediment samples were collected from shallow lakes, homogenized, and incubated for 18 days under stable laboratory conditions. The traditional method (1M) involved simple water filtration from incubated sediments. The so called “inverted funnel filtering” method (2M) includes an inverted funnel located above the sediment to trap zooplankton that passes through the funnel aperture, and the “levels filtering” method (3M) involves perforated plates above the sediment. The efficiency of each method was evaluated by analyzing the cumulative abundance and number of taxa in hatched total zooplankton, rotifers, and microcrustaceans, as well as the overall composition.

Results: The new proposed methods significantly favored higher abundances than 1M for total zooplankton and rotifers. Even more, 3M outperformed 2M in the case of microcrustacean hatching abundances.

Conclusions: Our findings suggest that despite all analyzed methods being suitable for studying zooplankton hatchings, the newly proposed methods incorporating internal structures to minimize sediment resuspension displayed increased capture efficiency.

Keywords

methods, ex situ experiments, resistance eggs, passive zooplankton, sediment

Resumo

Objetivo: Este estudo tem como objetivo analisar a eficiência de dois novos métodos para experimentos de eclosão de zooplâncton ex situ, comparados com um método tradicional. Ambos os métodos propostos foram especificamente projetados para minimizar a ressuspensão de sedimentos durante a amostragem de indivíduos eclodidos quando não há isolamento prévio dos ovos.

Métodos: Amostras de sedimentos foram coletadas de lagos rasos, homogeneizadas e incubadas por 18 dias em condições laboratoriais estáveis. O método tradicional (1M) envolveu uma simples filtração da água dos sedimentos incubados. O método chamado “filtragem por funil invertido” (2M) inclui um funil invertido localizado acima do sedimento para capturar zooplâncton que passasse pela abertura do funil, e o método “filtragem por níveis” (3M) envolveu placas perfuradas acima do sedimento. A eficiência de cada método foi avaliada analisando a abundância cumulativa e o número de táxons no zooplâncton total eclodido, rotíferos e microcrustáceos, bem como a composição geral.

Resultados: Os novos métodos propostos favoreceram significativamente uma maior abundância do que 1M para zooplâncton total e rotíferos. Além disso, 3M superou 2M no caso das capturas de eclosão de microcrustáceos.

Conclusões: Nossos resultados sugerem que, apesar de todos os métodos analisados serem adequados para estudar eclosões de zooplâncton, os novos métodos propostos que incorporam estruturas internas para minimizar a ressuspensão de sedimentos apresentaram maior eficiência de captura.

Palavras-chave

métodos, experimentos ex situ, ovos de resistência, zooplâncton passivo, sedimento

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Submitted date:
12/26/2023

Accepted date:
05/13/2024

Publication date:
07/22/2024

669e5f99a953956d0140ec02 alb Articles
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Acta Limnol. Bras. (Online)

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