Biological strategy to improve decomposition of organic matter in tilapia pond

Gustavo Ruschel Lopes; Hugo Mendes de Oliveira; Gabriel Fernandes Alves de Jesus; Maurício Laterça Martins; Carlos Henrique Araújo de Miranda Gomes; Thiago Soligo; José Luiz Pedreira Mouriño

doi:10.1590/S2179-975X8419

Short Research Note

Biological strategy to improve decomposition of organic matter in tilapia pond

Estratégia biológica para incremento da decomposição de matéria orgânica em viveiros de tilápia

Gustavo Ruschel Lopes; Hugo Mendes de Oliveira; Gabriel Fernandes Alves de Jesus; Maurício Laterça Martins; Carlos Henrique Araújo de Miranda Gomes; Thiago Soligo; José Luiz Pedreira Mouriño

http://dx.doi.org/10.1590/S2179-975X8419 Acta Limnol. Bras. (Online), vol.32, e27, 2020

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Abstract

Abstract: : Aim: The increment of decomposition of organic matter in sediment samples from Nile tilapia farms was evaluated with the introduction of Bacillus subtilis and B. licheniformis bacteria.

Methods: Sediment samples placed in 18L plastic boxes received single dose inoculum with the following concentrations: 1.21 x 10⁶ CFU g^-1 (equivalent to 75 g ha^-1), 2.41 x 10⁶ CFU g^-1 (equivalent to 150 g ha^-1), 4.82 x 10⁶ CFU g^-1 (equivalent to 300 g ha^-1) and 1.61 x 10⁷ CFU g^-1 (equivalent to 1000 g ha^-1), in addition to a control treatment with saline solution only. Organic matter content, total organic carbon (TOC) and oxidizable (OOC), total nitrogen (TN), ratios (TOC: N and OOC: N), clay content, pH in water, Shoemaker, McLean, Pratt index (SMP Index), phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg) contents, potential acidity (H + Al), cation exchange capacity (CEC) at pH 7.0, base saturation (V) and sum of bases (S).

Results: The values of OM showed significant difference, between the lowest values (treatments 75, 150 and 300 g ha^-1) and the highest value, (control treatment). TOC, OOC, NT and their relationships (TOC: N and OOC: N) showed significant differences between the mean values of the control treatment and the other treatments.

Conclusions: The addition of Bacillus subtilis and B. licheniformis bacteria increased the decomposition rate of organic matter in sediments samples from Nile tilapia farms.

Keywords

aquaculture, bioremediation, Bacillus subtilis, Bacillus licheniformis, sediment

Resumo

Resumo: : Objetivo: Foi avaliado o incremento da decomposição da matéria orgânica em amostras de sedimento de viveiros de criação de tilápias-do-Nilo com a introdução das bactérias Bacillus subtilis e B. licheniformis.

Métodos: Amostras de sedimento colocadas em caixas plásticas de 18L, receberam dose única de inóculos com as seguintes concentrações: 1,21 x 10⁶ UFC g^-1 (equivalente a 75 g ha^-1), 2,41x 10⁶ UFC g^-1 (equivalente a 150 g ha^-1), 4,82 x 10⁶ UFC g^-1 (equivalente a 300 g ha^-1) e 1,61x 10⁷ UFC g^-1 (equivalente a 1000 g ha^-1), além de um tratamento controle, com adição apenas de solução salina. Foram analisados teor de matéria orgânica, teores de carbono orgânico total (COT) e oxidável (COO), nitrogênio total (NT), relações de carbono: nitrogênio (COT:N e COO:N), teor de argila, pH em água, índce Shoemaker, McLean, Pratt (índice SMP), teores de fósforo (P), de potássio (K), de cálcio (Ca) e de magnésio (Mg), acidez potencial (H+Al), capacidade de troca catiônica (CTC) a pH 7,0 e saturação por bases (V).

Resultados: Os valores de MO apresentaram diferença significativa entre os menores valores (tratamentos 75, 150 e 300 g ha^-1) e o maior valor (tratamento controle). COT, COO, NT e suas relações (COT:N e COO:N) apresentaram diferenças significativas entre os valores médios do tratamento controle e os demais tratamentos.

Conclusões: A adição das bactérias Bacillus subtilis e B. licheniformis incrementou a taxa de decomposição de matéria orgânica em amostras de sedimento de viveiro de criação de tilápias-do-Nilo.

Palavras-chave

aquicultura, biorremediação, Bacillus subtilis, Bacillus licheniformis, sedimento

References

AMERICAN PUBLIC HEALTH ASSOCIATION – APHA. Standard methods for the examination of water and wastewater. 21st ed. Washington: APHA, 2005.

BOYD, C.E., WOOD, C.W. and THUNJAI, T. Aquaculture pond bottom soil quality management. Corvalis: PD/ACRSP, 2002.

BOYD, C.E., WOOD, C.W., CHANEY, P.L. and QUEIROZ, J.F. Role of aquaculture pond sediments in sequestration of annual global carbon emissions. Environmental Pollution, 2010, 158(8), 2537-2540. http://dx.doi.org/10.1016/j.envpol.2010.04.025. PMid:20537775.

CAMPOS, D.V.B., TEIXEIRA, P.C., PÉREZ, D.V. and SALDANHA, M.F.C. Acidez potencial do solo. In: P.C. TEIXEIRA, G.K. DONAGEMMA, A. FONTANA and W.G. TEIXEIRA, eds. Manual de métodos de análise de solo. Brasília: Embrapa, 2017, pp. 233-237.

COLDEBELLA, A., GENTELINI, A., PIANA, P., COLDEBELLA, P., BOSCOLO, W. and FEIDEN, A. Effluents from fish farming ponds: a view from the perspective of its main components. Sustainability, 2017, 10(1), 1-16. http://dx.doi.org/10.3390/su10010003.

DE MARCO, E.G., HECK, K., MARTOS, E.T. and VAN DER SAND, S.T. Purification and characterization of a thermostable alkaline cellulase produced by Bacillus licheniformis 380 isolated from compost. Annals of the Brazilian Academy of Sciences, 2017, 89(3, Suppl.), 2359-2370. http://dx.doi.org/10.1590/0001-3765201720170408. PMid:29044330.

DEGERING, C., EGGERT, T., PULS, M., BONGAERTS, J., EVERS, S., MAURER, K. and JAEGER, K. Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and heterologous signal peptides. Applied and Environmental Microbiology, 2010, 76(19), 6370-6376. http://dx.doi.org/10.1128/AEM.01146-10. PMid:20709850.

FOOD AND AGRICULTURE ORGANIZATION – FAO. The state of world fisheries and aquaculture 2018: meeting the sustainable development goals. Rome: FAO, 2018.

IQBAL, S.A. and REHMAN, A. Characterization of lipase from Bacillus subtilis I-4 and its potential use in oil contaminated wastewater. Brazilian Archives of Biology and Technology, 2015, 58(5), 789-797. http://dx.doi.org/10.1590/S1516-89132015050318.

KARIGAR, C.S. and RAO, S.S. Role of microbial enzymes in the bioremediation of pollutants: a review. Enzyme Research, 2011, 2011, 805187. http://dx.doi.org/10.4061/2011/805187. PMid:21912739.

MADIGAN, M.T., MARTINKO, J.M., STAHL, D.A. and CLARK, D.P. Brock biology of microorganisms. 13th ed. San Francisco: Benjamin Cummings, 2010.

MEDEIROS, F. Anuário Peixe BR da Piscicultura 2019. São Paulo: Associação Brasileira da Piscicultura, 2019.

NAVARRO, A.F., CEGARRA, J., ROIG, A. and GARCIA, D. Relationships between organic matter and carbon contents of organic wastes. Bioresource Technology, 1993, 44(3), 203-207. http://dx.doi.org/10.1016/0960-8524(93)90153-3.

RAUL, D., BISWAS, T., MUKHOPADHYAY, S., KUMAR DAS, S. and GUPTA, S. Production and partial purification of alpha amylase from Bacillus subtilis (MTCC 121) using solid state fermentation. Biochemistry Research International, 2014, 2014, 568141. http://dx.doi.org/10.1155/2014/568141. PMid:24672727.

SILVA, F.C. Manual de análises químicas de solos, plantas e fertilizantes. 2. ed. Brasília: Embrapa Informação Tecnológica, 2009.

TEDESCO, M.J., GIANELLO, C., BISSANI, C.A., BOHNEN, H. and VOLKWEISS, S.J. Análise de solo, plantas e outros materiais. 2. ed. Porto Alegre: Departamento de Solos, Universidade Federal do Rio Grande do Sul, 1995.

TEIXEIRA, P.C., DONAGEMMA, G.K., FONTANA, A. and TEIXEIRA, W.G. Manual de métodos de análise de solo. Brasília: Embrapa, 2017.

TRACHUK, L.A., REVINA, L.P., SHEMYAKINA, T.M., CHESTUKHINA, G.G. and STEPANOV, V.M. Chitinases of Bacillus licheniformis B-6839: isolation and properties. Canadian Journal of Microbiology, 1996, 42(4), 307-315. http://dx.doi.org/10.1139/m96-046.

YI, P., PAI, C. and LIU, J. Isolation and characterization of a Bacillus licheniformis strain capable of degrading zearalenone. World Journal of Microbiology & Biotechnology, 2011, 27(5), 1035-1043. http://dx.doi.org/10.1007/s11274-010-0548-7.

YOON, K.-H. Cloning of the Bacillus subtilis AMX-4 Xylanase gene and characterization of the gene product. Journal of Microbiology and Biotechnology, 2009, 19(12), 1514-1519. http://dx.doi.org/10.4014/jmb.0907.07004. PMid:20075612.

Submitted date:
09/30/2019

Accepted date:
08/21/2020

Publication date:
11/25/2020

Biological strategy to improve decomposition of organic matter in tilapia pond

Estratégia biológica para incremento da decomposição de matéria orgânica em viveiros de tilápia

Gustavo Ruschel Lopes; Hugo Mendes de Oliveira; Gabriel Fernandes Alves de Jesus; Maurício Laterça Martins; Carlos Henrique Araújo de Miranda Gomes; Thiago Soligo; José Luiz Pedreira Mouriño

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