Acta Limnologica Brasiliensia
https://actalb.org/article/doi/10.1590/S2179-975X3321
Acta Limnologica Brasiliensia
Original Article

Effects of formaldehyde preservation time on the length-weight relationship of the ubiquitous neotropical cladoceran Ceriodaphnia silvestrii

Efeito do tempo de preservação por formaldeído na relação peso-comprimento do ubíquo cladócero neotropical Ceriodaphnia silvestrii

Rafael Lacerda Macêdo; Lorena Pinheiro-Silva; Adriana Lamanna Puga; Gabriel Klippel; Betina Kozlowsky-Suzuki; Odete Rocha; Christina Wyss Castelo Branco

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Abstract

Abstract: : Aim: In this study, the effect of preservation time on total body length and dry weight of adult specimens of the neotropical cladoceran Ceriodaphnia silvestrii preserved with 4% formalin solution were examined.

Methods: The relationship between these variables was examined under increasing gradual time effects (i.e. 7, 30, and 60 days) after preservation using linear models and analysis of variance.

Results: Total body length did not statistically differ between fresh and preserved cladocerans at any preservation time, whereas dry weight was drastically reduced with increasing preservation time, with 15, 47 and 57% weight losses. Length-weight relationships were significantly and positively related in all treatments, though higher values of slope were found for fresh and 7 days samples.

Conclusions: We highlight that, for Ceriodaphnia silvestrii, the use of the formalin solution as a preservation fixative is not adequate when the major interest is biomass estimation. Also, we recommend that dry weight estimations from preserved samples should be done as soon as possible. Finally, considering the preservation losses and intra-specific composition of organisms, the application of correction factors is advised since preserved samples are important in the evaluation of long-term changes of biological communities.

Keywords

body length, Daphnidae, dry weight, fresh weight, secondary productivity

Resumo

Resumo: : Objetivo: Neste estudo, examinamos o efeito do tempo de preservação no comprimento total do corpo e no peso seco de espécimes adultos do cladócero neotropical Ceriodaphnia silvestrii fixados com solução de formalina a 4%.

Métodos: A relação entre essas variáveis foi examinada sob efeito crescente do tempo (7, 30 e 60 dias) após a preservação usando modelos lineares e análise de variância.

Resultados: O comprimento corporal total não diferiu estatisticamente entre os cladóceros não-preservados e preservados, enquanto o peso seco foi reduzido com o aumento do tempo de preservação, resultando 15, 47 e 57% de perda de peso, respectivamente. As relações peso-comprimento foram significativamente positivas em todos os tratamentos, embora valores mais elevados tenham sido encontrados em amostras frescas e de 7 dias.

Conclusões: Destacamos que, para Ceriodaphnia silvestrii, o uso da solução de formalina como método de preservação não é adequado quando o principal interesse é a estimativa de biomassa. Recomendamos que as estimativas de peso seco de amostras preservadas sejam feitas o mais rápido possível. Por fim, considerando as perdas atribuídas à preservação e às variações intraespecíficas da biomassa dos organismos, recomenda-se a aplicação de fatores de correção uma vez que amostras preservadas são importantes na avaliação de mudanças de longo prazo nas comunidades biológicas.
 

Palavras-chave

tamanho corporal, Daphnidae, peso seco, peso fresco, produção secundária

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Submitted date:
04/30/2021

Accepted date:
09/26/2021

Publication date:
04/28/2022

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