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Acta Limnologica Brasiliensia
https://actalb.org/article/doi/10.1590/S2179-975X2122
Acta Limnologica Brasiliensia
Original Article

Screening of cyanotoxin producing genes in Ecuadorian freshwater systems

Triagem de genes produtores de cianotoxinas em sistemas de água doce do Equador

Isabel Ballesteros; Sara de la Cruz; Michelle Rojas; Génesis Salazar; Miguel Martínez-Fresneda; Pablo Castillejo

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Abstract

Abstract:: Aim: This study pursued the detection of cyr and mcy genes to assess the presence of cylindrospemopsin (CN) and microcystin (MC) potential producers in Ecuadorian water bodies.

Methods: Environmental DNA (eDNA) was extracted from eight water bodies and one wastewater treatment plant (WWTP) from Ecuador. A nested PCR was designed to amplify mcyB, cyrE, and cyrJ genes in these environmental samples. PCR products were sequenced and blasted against GenBank database.

Results: Potential CN producers were found in seven water bodies and the WWTP. cyrE amplification revealed three variants belonging to Raphidopsis and Aphanizmenon species and one for cyrJ with around 90% identity with Raphidiopsis and Oscillatoria species. Four water bodies presented the same variant for mcyB similar to Microcystis panniformis with 99% of identity.

Conclusions: This study contributes new data on the presence of toxic cyanobacteria strains and provides new molecular tools to assess cyanotoxin hazards in Ecuadorian water bodies.

Keywords

cylindrospermopsin, microcystin, nested PCR, Andes, Amazon

Resumo

Resumo:: Objetivo: Este estudo buscou a detecção de genes cyr e mcy para avaliar a presença de potenciais produtores de cilindrospemopsina (CN) e microcistina (MC) em corpos d'água equatorianos.

Métodos: O DNA ambiental (eDNA) foi extraído de oito corpos d'água e uma estação de tratamento de águas residuais (ETAR) do Equador. Uma “nested” PCR foi projetada para amplificar os genes mcyB, cyrE e cyrJ nessas amostras ambientais. Os produtos de PCR foram sequenciados e pesquisados no banco de dados GenBank.

Resultados: Potenciais produtores de CN foram encontrados em sete corpos d'água e na ETAR. A amplificação de cyrE revelou três variantes pertencentes à espécies dos gêneros Raphidopsis e Aphanizomenon e uma para cyrJ com cerca de 90% de identidade com espécies dos gêneros Raphidiopsis e Oscillatoria. Quatro corpos d'água apresentaram a mesma variante para mcyB semelhante a Microcystis panniformis com 99% de identidade.

Conclusões: Este estudo contribui com novos dados sobre a presença de cianobactérias tóxicas e fornece novas ferramentas moleculares para avaliar os riscos de cianotoxinas em corpos d'água equatorianos.
 

Palavras-chave

cilindrospermopsina, microcistina, nested PCR, Andes, Amazônia

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Submitted date:
03/09/2022

Accepted date:
09/28/2022

Publication date:
10/11/2022

6345ae94a953951224285883 alb Articles
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Acta Limnol. Bras. (Online)

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